Detection of NDM-1 producing Klebsiella pneumoniae ST15 and ST147 in Iran during 2019–2020

Abstract Carbapenems are employed to treat infections caused by Gram-negative bacteria including Klebsiella pneumoniae . This research is aimed perform phenotypic detection of ?-lactamases and molecular characterization NDM-1 positive K. isolates. Another objective investigate producing among children in Iran. From 2019 2020, altogether 60 isolates were acquired from various patients certain Iranian hospitals. Antimicrobial susceptibility testing was performed disk diffusion broth microdilution methods. In addition, mCIM eCIM used confirm the production carbapenemases metallo-beta-lactamases (MBLs), respectively. Detection resistance genes namely, bla , IMP VIM KPC OXA-48-like CTX-M SHV TEM mcr-1 PCR confirmed DNA sequencing. Multilocus sequence typing (MLST) determine strains. According findings, highest rate carbapenem detected against doripenem 83.3% (50). Moreover, 31.7% (19) resistant colistin. Further above, 80% (48) carbapenemase-producing them 46.7% (28) MBL 33.3% (20) serine ?-lactamase producer. results, 14 produced Remarkably, four children. these clonally related as determined MLST (ST147, ST15). Altogether ten ST147 ST15. Based on emergence NDM-producing worrying hence, it necessary develop a comprehensive program control antibiotic country.